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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
28/08/2014 |
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Data da última atualização: |
02/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PERINI, P.; PASQUALI, G.; MARGIS-PINHEIRO, M.; OLIVEIRA, P. R. D. de; REVERS, L. F. |
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Afiliação: |
Pâmela Perini; Giancarlo Pasquali; Márcia Margis-Pinheiro; PAULO RICARDO DIAS DE OLIVEIRA, CNPUV; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Reference genes for transcriptional analysis of flowering and fruit ripening stages in apple (Malus 3 domestica Borkh.). |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Molecular Breeding, mar. 2014. |
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Idioma: |
Inglês |
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Notas: |
DOI 10.1007/s11032-014-0078-3 |
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Conteúdo: |
Apple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are valuable scientific tools to better understand the gene expression changes behind important silvicultural and nutritional traits. However, the accuracy of techniques employed to access gene expression is dependent on the evaluation of stable reference genes for data normalization to avoid statistical significance undue or incorrect conclusions. The objective of this work was to select the best genes to be used as references for gene expression studies in apple trees by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Vegetative and reproductive tissues of the apple ??Gala?? cultivar were evaluated during their seasonal cycle of growth and dormancy. The expression of 23 traditional housekeeping genes or genes suggested as constitutive by microarray data was investigated. Tested combinations of primers allowed the specific amplification and the generation of suitable efficiency curves for gene expression studies by RT-qPCR. Gene stability was determined by two different statistical descriptors, geNorm and Norm-Finder. The known variable PAL gene expression was used to validate selected normalizers. Results obtained allowed us to conclude that MDH, SAND, THFS, TMp1 and WD40 are the best reference genes to accurately normalize the relative transcript abundances using RT-qPCR in various tissues of apple. MenosApple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are valuable scientific tools to better understand the gene expression changes behind important silvicultural and nutritional traits. However, the accuracy of techniques employed to access gene expression is dependent on the evaluation of stable reference genes for data normalization to avoid statistical significance undue or incorrect conclusions. The objective of this work was to select the best genes to be used as references for gene expression studies in apple trees by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Vegetative and reproductive tissues of the apple ??Gala?? cultivar were evaluated during their seasonal cycle of growth and dormancy. The expression of 23 traditional housekeeping genes or genes suggested as constitutive by microarray data was investigated. Tested combinations of primers allowed the specific amplification and the generation of suitable efficiency curves for gene expression studies by RT-qPCR. Gene stability was determined by two different statistical descriptors, geNorm and Norm-Finder. The known variable PAL gene expression was used to validate selected normalizers. Results obtained allowed us to conclude that MDH, SAND, THFS, TMp1 and WD40 are the best reference genes to accurately normalize the relative transcript abundances u... Mostrar Tudo |
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Palavras-Chave: |
Expressão genética; Gala; Genes de referencia; RT-qPCR. |
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Thesagro: |
Genetica vegetal; Maçã. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107436/1/Perini2014-Reference-Genes-Apple.pdf
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Marc: |
LEADER 02287naa a2200253 a 4500
001 1993623
005 2019-04-02
008 2014 bl uuuu u00u1 u #d
100 1 $aPERINI, P.
245 $aReference genes for transcriptional analysis of flowering and fruit ripening stages in apple (Malus 3 domestica Borkh.).$h[electronic resource]
260 $c2014
500 $aDOI 10.1007/s11032-014-0078-3
520 $aApple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are valuable scientific tools to better understand the gene expression changes behind important silvicultural and nutritional traits. However, the accuracy of techniques employed to access gene expression is dependent on the evaluation of stable reference genes for data normalization to avoid statistical significance undue or incorrect conclusions. The objective of this work was to select the best genes to be used as references for gene expression studies in apple trees by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Vegetative and reproductive tissues of the apple ??Gala?? cultivar were evaluated during their seasonal cycle of growth and dormancy. The expression of 23 traditional housekeeping genes or genes suggested as constitutive by microarray data was investigated. Tested combinations of primers allowed the specific amplification and the generation of suitable efficiency curves for gene expression studies by RT-qPCR. Gene stability was determined by two different statistical descriptors, geNorm and Norm-Finder. The known variable PAL gene expression was used to validate selected normalizers. Results obtained allowed us to conclude that MDH, SAND, THFS, TMp1 and WD40 are the best reference genes to accurately normalize the relative transcript abundances using RT-qPCR in various tissues of apple.
650 $aGenetica vegetal
650 $aMaçã
653 $aExpressão genética
653 $aGala
653 $aGenes de referencia
653 $aRT-qPCR
700 1 $aPASQUALI, G.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aOLIVEIRA, P. R. D. de
700 1 $aREVERS, L. F.
773 $tMolecular Breeding, mar. 2014.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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| Registros recuperados : 40 | |
| 4. |  | PASSAIA, G.; SBEGHEN, F.; MARGIS-PINHEIRO, M.; REVERS, L. F. Avaliação da expressão e identificação de genes diferencialmente regulados durante o desenvolvimento do fruto das cultivares de uva Isabel e Isabel Precoce. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 5., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 1., 2007, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2007. p. 47. (Embrapa Uva e Vinho. Documentos, 63). Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 12. | | FALAVIGNA, V. S.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Análise funcional e potencial biotecnológico do gene DHN11 de macieira (Malus x domestica Borkh.) In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 82. (Embrapa Uva e Vinho. Documentos, 99)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 14. | | PASSAIA, G.; MARGIS-PINHEIRO, M.; FIALHO, F. B.; SBEGHEN, F.; PORTO, D. D.; REVERS, L. F. Characterization of an early berry development grapevine somatic variant (Vitis labrusca L. cv. Isabel Precoce). American Journal of Plant Sciences, v. 5, n. 26, p. 3848-3865, Dec. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 19. | | MALABARBA, J.; BUFFON, V.; FRÂNCIO, L.; MARASCHIN, F. S.; PASQUALI, G.; MÁRGIS-PINHEIRO, M. N.; REVERS, L. F. Avaliação da função de VvAGL11 na morfogênese da semente da videira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 46. (Embrapa Uva e Vinho. Documentos, 99)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 40 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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